Human CFB PharmaGenie ELISA Kit (SBRS0412)

The Assay Genie Human CFB ELISA Kit is built around Complement Factor B (CFB), the 93 kDa zymogen that drives the alternative complement pathway. Factor D cleaves CFB into the Ba fragment and the catalytic Bb subunit, which assembles with C3b into the C3 convertase C3bBb that amplifies complement activation. Because gain-of-function variants and dysregulated alternative pathway activity are linked to age-related macular degeneration, atypical hemolytic uremic syndrome, and C3 glomerulopathy, this kit supports immunology, nephrology, and ophthalmology researchers measuring circulating factor B, validating disease-associated variants, and tracking pharmacodynamic responses to complement-targeted therapeutics.

The pre-coated capture antibody is human-specific, with biotinylated detection and HRP-based colorimetric readout for straightforward quantitation. Each well takes 100 uL of sample, giving laboratories a reproducible workflow for cell culture supernatant, plasma, and serum specimens in biomarker validation, pathway activation profiling, and complement therapeutics research.