Human Ceruloplasmin ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed to accurately measure ceruloplasmin levels in human samples including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. Using a colorimetric, double-antibody Sandwich ELISA format, the kit provides reproducible results for research into iron metabolism, antioxidant defense, and the regulation of inflammatory responses, where ceruloplasmin plays a key physiological role.

The assay has a stated sensitivity of 75 pg/mL and a working range of 125-8000 pg/mL. Reported linearity falls between 81% and 101% across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with recovery averaging 93% (serum 89-105%, EDTA plasma 85-98%, heparin plasma 86-101%). The protocol uses a biotin-labeled detection antibody with HRP-Streptavidin (SABC), TMB substrate development read at 450 nm, and reagents are stored at 2-8°C for 12 months. Abnormal ceruloplasmin levels have been linked to conditions such as Wilson's disease, Alzheimer's disease, and cardiovascular disorders, making this kit a useful biomarker tool for related research.