Human Ceramide glucosyltransferase (UGCG) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise and accurate measurement of Ceramide glucosyltransferase (UGCG) levels in human samples, with reactivity limited to human specimens and a colorimetric detection method. It is for research use only.

The kit accepts serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. According to the manufacturer datasheet, the detection range is 0.78-50ng/mL, sensitivity is 0.34ng/mL, intra-assay CV is 4.6%, and inter-assay CV is 8.6%. Reported recovery is 87% (range 81-93%) for serum and 89% (range 83-95%) for plasma. Specificity is defined against natural and recombinant human Ceramide glucosyltransferase, and the listed research area is cardiovascular.

UGCG catalyzes the first glycosylation step in glycosphingolipid biosynthesis, transferring glucose to ceramide, and may also serve as a "flippase". Dysregulation of UGCG activity has been implicated in skin disorders, neurodegenerative diseases, and metabolic disorders, supporting its use as a potential biomarker. The UniProt entry is Q16739, and the protein localises to the Golgi apparatus membrane as a multi-pass membrane protein. Alternative names include GLCT-1, Glucosylceramide synthase, UDP-glucose ceramide glucosyltransferase, UDP-glucose:N-acylsphingosine D-glucosyltransferase, and GCS.