Human CDX2 (Homeobox protein CDX-2) ELISA Kit (HUFI04784)

The Assay Genie CDX2 ELISA Kit enables quantitative measurement of Homeobox protein CDX-2, a caudal-type homeobox transcription factor central to intestinal epithelial differentiation. Investigators apply this kit in research on colorectal cancer, Barrett's esophagus, intestinal metaplasia, and gastroenterology, where CDX2 expression serves as a differentiation marker. The kit supplies a pre-coated microplate, biotinylated detection reagent, assay buffers, and a set of protein standards calibrated across 0.16–10 ng/mL, sufficient for 96 individual determinations. This working range supports sensitive quantification of CDX2 in 50 µL sample volumes of cell lysate and tissue homogenate from patient cohort studies. Reconstituted standards should be aliquoted and frozen at −20 °C to limit freeze-thaw cycles and preserve antibody binding activity across repeat experiments. The enclosed protocol recommends running all measurements in duplicate within the same plate to minimise inter-assay variation, with short-term incubation at room temperature (18–25 °C) during the assay.