Human Cdc7 Kinase ELISA Kit (HUFI07975)

Cdc7 kinase is a serine/threonine kinase that phosphorylates MCM2 and other components of the replicative helicase complex, licensing origins of replication and driving the G1/S phase transition. Because Cdc7 expression and activity are frequently dysregulated in cancer cells, the protein has become a focal target of interest for oncology drug discovery and replication-stress research.

The Human Cdc7 Kinase ELISA Kit from Assay Genie is configured for 96 individual reactions, supporting medium-throughput studies of Cdc7 protein abundance in human specimens. Each kit includes the necessary reagents and calibrated standards to perform the immunoassay, and the chromogenic endpoint can be quantified on any standard microplate reader equipped for absorbance measurement.

Researchers apply the kit to quantify endogenous Cdc7 levels in cell lysates, tissue homogenates, and conditioned media from cultured human cell lines. Because Cdc7 sits at the intersection of DNA replication initiation and checkpoint control, the kit is used in studies of replication timing, S-phase entry, and the cellular response to DNA-damaging agents. Reagents should be refrigerated on arrival; reconstituted components are typically aliquoted and frozen to minimize repeated freeze-thaw cycles. For research use only.