Human CD95/FAS ELISA Kit

A high-quality sandwich ELISA from Assay Genie designed for the quantitative detection of Human FAS/CD95 (Factor Related Apoptosis) levels across a broad range of biological samples. The assay uses a colorimetric detection method with a pre-coated microplate and a biotinylated detection antibody combined with an Avidin-Horseradish Peroxidase (HRP) conjugate workflow, providing robust performance for both research and clinical applications.

The kit is intended for use with serum, plasma and other biological fluids (100 µL sample volume) and shows no significant cross-reactivity or interference between Human FAS/CD95 and analogues. Sensitivity is reported at 0.08 ng/mL, with a detection range of 0.16–10 ng/mL and recovery of 80–120%. Linearity, recovery and intra-/inter-assay precision data have been validated for serum, EDTA plasma and cell culture media. Storage conditions for the kit are 2–8 °C. Research areas include cancer, cell biology and immunology, and the recognised UniProt identifier is P25445.