Human CD80/B7-1 PharmaGenie ELISA Kit (SBRS0398)

The Assay Genie CD80/B7-1 quantitative immunoassay provides a robust absorbance-based method for measuring soluble human B7-1 protein in research samples. Compatible sample matrices include cell culture supernatants, serum or plasma, with a recommended two-fold dilution factor for unknown specimens. Each kit supplies 96 assays per microplate together with a pre-coated plate, detection antibody, recombinant reference standards, substrate solution, and wash buffer, supporting standard plate-reader workflows across immunology, oncology, and autoimmune disease research programs.

The CD80 molecule functions as a critical co-stimulatory ligand, engaging CD28 on T lymphocytes to promote activation while also binding CTLA-4 to deliver inhibitory signals. Quantifying circulating CD80/B7-1 supports investigations into cancer immunotherapy efficacy, transplant rejection monitoring, chronic inflammatory conditions, and biomarker discovery efforts targeting immune checkpoint pathways. Reconstituted standard material may be stored frozen at -80 °C for up to one week, and unopened kits remain stable refrigerated for six months; researchers should avoid repeated freeze-thaw cycles to preserve reagent performance and reproducibility. The whole kit can be kept at -20 °C for up to one year when long-term storage is required.

For research use only. Not for use in diagnostic or therapeutic procedures.