Human CD40L/TNFSF5 ELISA Kit

Manufactured by Assay Genie, this ELISA kit is designed for the quantitative detection of CD40L (also referred to as TNFSF5) in human samples. It uses a sandwich ELISA, double antibody format with colorimetric readout, and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. The assay has a sensitivity of 37.5 pg/mL and a dynamic range of 62.5-4000 pg/mL, with unopened components stored at 2-8°C for up to 12 months.

Validated linearity and recovery data are provided for serum, EDTA plasma, and heparin plasma across dilution factors. The procedure involves reagent and plate equilibration, a 37°C primary incubation, biotinylated detection antibody binding, HRP-streptavidin binding, TMB substrate development in the dark, and absorbance measurement at 450 nm. Sample preparation guidance covers serum, plasma, tissue homogenates, cell culture supernatants, and cell lysates.

CD40L (TNFSF5) is a key protein involved in immune responses and inflammation, contributing to immune cell activation and immune system regulation. Dysregulation has been linked to inflammatory diseases, autoimmune disorders, and cancers, supporting its relevance as a biomarker for research into disease processes and therapeutic targets.