Human CD30/TNFRSF8 ELISA Kit

From Assay Genie, this Human CD30/TNFRSF8 ELISA Kit is a sandwich (double antibody) colorimetric ELISA designed for the quantitative measurement of TNFRSF8 (tumor necrosis factor receptor superfamily member 8) in human samples. The kit is reactive with human samples and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples as input. Readout is by absorbance at 450 nm following TMB substrate development and stop solution, and the kit includes validated Bradford Reagent to support total protein quantification for sample normalization.

Reported assay characteristics include a range of 0.781–50 ng/mL and a sensitivity of 0.469 ng/mL. Recovery is reported for serum (range 90–98%, average 94%), EDTA plasma (range 89–100%, average 95%), and heparin plasma (range 94–105%, average 99%). Linearity at 1:2, 1:4 and 1:8 dilutions is reported for serum (86–103%, 82–94%, 87–100%), EDTA plasma (88–103%, 87–101%, 80–99%) and heparin plasma (86–98%, 82–101%, 85–100%). The assay procedure follows a sandwich ELISA workflow with incubation at 37 °C, biotin-labeled detection antibody binding, HRP-streptavidin binding, TMB color development in the dark, and absorbance measurement at 450 nm after stop solution is added. Kit components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), Bradford reagent, plate sealer, and a technical manual. The kit is intended for research use only.