Human CD28 PharmaGenie ELISA Kit (SBRS0373)

CD28 is a disulfide-linked homodimeric glycoprotein expressed on most T lymphocytes, where it serves as the principal co-stimulatory receptor for naive and memory T cells. Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) on antigen-presenting cells synergizes with TCR signaling to drive IL-2 transcription, clonal expansion, and survival, making accurate quantification of soluble or shed CD28 valuable in transplant immunology, autoimmune disease research, and vaccine adjuvant studies.

Assay Genie supplies this reagent set as a complete microplate with all buffers, capture antibodies, detection antibodies, and lyophilized standards required to measure native human CD28 in conditioned medium from activated lymphocytes, EDTA-treated whole-blood derivatives, and donor-matched biofluid specimens. Researchers commonly apply the assay to monitor T-cell activation kinetics in PBMC cultures, characterize immune phenotypes in patient cohorts, and validate candidate immunomodulators in vitro. Reconstituted standards should be stored at -20 °C and protected from light, while unused strip wells can be kept refrigerated for repeated use within the stated shelf life.