Human CD276 antigen (CD276) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of human CD276 (also known as B7-H3) in biological samples. CD276 is described as a critical immune checkpoint protein that plays a vital role in regulating immune responses and tumor microenvironment interactions, with dysregulation implicated in cancer and autoimmune disorders, making it a valuable biomarker for studying disease progression and potential therapeutic interventions.

The kit is reactive with human samples and validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. It operates as a colorimetric sandwich ELISA with a detection range of 0.78–50 ng/mL and a sensitivity of 0.21 ng/mL. Reported precision values include an intra-assay CV of 5.2% and an inter-assay CV of 9.1%.

Linearity has been assessed in serum, EDTA plasma and heparin plasma across dilution ratios of 1:2, 1:4, 1:8 and 1:16, while recovery in serum averages 91% (range 85–97%) and in plasma averages 93% (range 87–99%). The kit is intended for research use only and is suited to cancer research applications.