Human CD27 (T-cell activation antigen CD27) ELISA Kit (HUFI04566)

Manufactured by Assay Genie, this quantitative immunoassay enables precise measurement of human CD27 (TNFRSF7), a 55 kDa transmembrane glycoprotein belonging to the TNF receptor superfamily. The assay exploits the CD27-CD70 receptor-ligand axis, a critical costimulatory pathway driving T-cell expansion, germinal center formation, and plasma cell differentiation. Researchers apply this kit to monitor soluble CD27 shedding in samples from patients with systemic lupus erythematosus, B-cell chronic lymphocytic leukemia, multiple sclerosis, and HIV infection, where elevated titers correlate with disease activity. Additional applications include vaccine immunogenicity profiling, NK-cell activation studies, and characterization of CAR-T cell products. Each kit is supplied with a pre-coated microplate, detection antibody, standards, and buffers, supporting longitudinal immunology investigations and biomarker discovery workflows in translational research programs. The reagents are intended for research use only and support detection of native human CD27 protein from cell culture supernatants and clinical sample matrices.