Human CD25/IL2 R alpha SuperSet ELISA Kit

A colorimetric ELISA from Assay Genie is configured for the detection of human CD25 (IL-2 receptor subunit alpha). Designed as a DIY-format kit aimed at pharmaceutical and biotechnology research, it is positioned as a tool for immunology work and accepts serum, plasma, and cell culture supernatant samples, requiring a sample volume of 100 µl. According to the manufacturer, the assay recognises both natural and recombinant human soluble IL-2R, with no cross-reactivity reported against other human soluble receptors.

Quantitative performance is characterised by an assay range of 68.75 pg/ml to 2220 pg/ml and a sensitivity of 8 pg/ml. The complete protocol, from sample addition to final read-out, is described as taking 3 hours 45 minutes. Each kit supplies a biotinylated detection antibody paired with a capture antibody, one standard per plate, streptavidin-HRP, TMB substrate, and a detailed procedure including buffer composition. The reagents are configured to detect analytes in cell culture supernatants as well as in more complex matrices such as serum and plasma.