Human CD200R1 (CD200 Receptor 1) ELISA Kit  (HUES03224)

The Assay Genie sandwich ELISA kit is built around a pre-coated 96-well microplate, with biotinylated detection antibody and streptavidin-HRP conjugate supplied as separate components. Each kit supports a standard curve generated from a serial dilution of the reconstituted lyophilized standard, generally yielding a working range of approximately 0.16 to 10 ng/mL, with intra-assay CV reported below 8% and inter-assay CV below 10%. The protocol typically requires 100 µL of sample per well, a 2.5 hour total incubation time at room temperature, and a final TMB substrate step that is stopped with 50 µL of 2 N sulfuric acid before absorbance is read at 450 nm with a 570 nm reference correction.

Dried reagents should be stored at 2-8 degrees C, the sealed plate pouch kept desiccated and protected from light, and working dilutions of standard and samples prepared fresh to avoid freeze-thaw degradation. The kit is validated for human serum collected in standard clot tubes, EDTA plasma, heparin plasma, and cell culture supernatants, with dilution buffer recommended for samples expected to fall outside the standard curve. Researchers studying multiple sclerosis, glioma, sepsis, and graft-versus-host disease have used this reagent to monitor CD200R1 expression in circulation and cerebrospinal fluid, complementing flow cytometry and immunohistochemistry readouts. For research use only, not intended for diagnostic procedures.