Human CD200 (Cluster of Differentiation 200) ELISA Kit  (HUES03345)

Beyond the sandwich immunoassay format, the Assay Genie CD200 ELISA Kit is calibrated against a recombinant human CD200 standard, producing a typical standard curve spanning roughly 15.6 to 1000 pg/mL, which permits quantitation of physiological and disease-elevated CD200 levels. The capture antibody is a pre-titered monoclonal antibody pre-coated onto the microplate wells, and detection is achieved with a biotinylated secondary antibody paired with streptavidin-HRP; absorbance is read at 450 nm with a 570 nm reference correction. Unopened kits are stored at 2 to 8 °C, while reconstituted standards and the streptavidin-HRP concentrate should be aliquoted and frozen at -20 °C to limit freeze-thaw cycles. Researchers commonly apply this kit to serum and plasma biomarker discovery in autoimmune cohorts, to tumor microenvironment profiling where CD200 expression on tumor cells correlates with myeloid-derived suppressor cell activity, and to neuroinflammation models, including microglial regulation studies. Performance is validated for cell culture supernatant samples as well, provided matrices are confirmed by spiking recovery. Each 96-well plate is supplied with pre-coated wells, biotinylated detection antibody, streptavidin-HRP, standards, wash buffer, substrate solution, and stop solution; the protocol typically runs in under 4 hours from sample addition to read.