Human CD137/TNFRSF9 ELISA Kit

Manufactured by Assay Genie, this ELISA kit provides a sensitive and reproducible method for the quantitative detection of human CD137 (TNFRSF9) in research samples. CD137 is a key immune-regulatory protein involved in T-cell activation and inflammatory responses, making measurement of its levels valuable for studies of immune system function, autoimmune disease, cancer immunotherapy, and host defence against pathogens. The kit employs a sandwich ELISA format with double antibody detection and a colorimetric readout.

The assay is validated for human reactivity and accepts a broad range of sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Performance specifications include a sensitivity of 18.75 pg/mL and a measurable range of 31.25–2000 pg/mL. Components are supplied for storage at 2–8°C for up to 12 months. Reported linearity for serum, EDTA plasma, and heparin plasma falls between 82% and 105% across dilutions up to 1:8, and recovery across the same sample types ranges from 88% to 105% with averages of 96–100%.

The workflow consists of an initial 90-minute incubation at 37°C for antigen binding, followed by 60 minutes with a biotin-labelled detection antibody, a 30-minute HRP-streptavidin binding step, and 10–20 minutes of TMB colour development in the dark before stop solution is added and absorbance is read at 450 nm. Detailed preparation guidance is provided for each sample type, including centrifugation conditions for serum, plasma, tissue homogenates, cell culture supernatants, and cell lysates.