Human CD 59 PharmaGenie ELISA Kit (SBRS0391)

The CD59 glycoprotein, also known as protectin, is a GPI-anchored cell-surface complement regulator that prevents the assembly of the terminal membrane attack complex (MAC) on host cells. Assaying circulating or shed CD59 levels gives researchers a quantitative window into complement dysregulation, with particular value in studies of paroxysmal nocturnal hemoglobinuria (PNH), autoimmune hemolysis, transplant rejection research, inflammatory disease models, and immune evasion in tumor biology.

Built for human sample work, this colorimetric ELISA Kit from Assay Genie is calibrated to quantify CD59 from cell culture supernatant, plasma, and serum specimens — the standard matrices used in complement and immunology laboratories. Each kit is configured for 96 assays, suiting throughput needs for cohort screening, longitudinal sampling, and routine bench evaluation. The protocol produces a signal read on a standard microplate reader, and the kit is supplied in a pack format ready for laboratory use.

Typical applications include biomarker profiling in complement-mediated disease studies, validation of CD59 expression changes in cell-based assays, and correlation with glycosylphosphatidylinositol (GPI) anchor deficiency investigations. Researchers commonly pair CD59 measurements with CD55 and CD46 assays to build a more complete picture of cell-surface complement regulation, since these three regulators cover different checkpoints of the complement cascade.

Storage and handling guidance for this Assay Genie kit follows standard refrigerated ELISA practice to preserve antibody and standard reagent activity; bring all components to room temperature before initiating the run, and consult the enclosed product insert for detailed preparation, dilution series, and assay procedure notes. For research use only.