Human CCNB1/Cyclin B1 ELISA Kit

From Assay Genie, this Human CCNB1/Cyclin B1 ELISA Kit is a colorimetric sandwich ELISA built on a double-antibody format for the quantitative measurement of cyclin B1 in human samples. The assay accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, supporting a range of cell-cycle research applications.

Reported performance figures include a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Spiked recovery was 88-104% (average 96%) in serum, 90-105% (average 99%) in EDTA plasma, and 88-99% (average 95%) in heparin plasma. Linearity at 1:2, 1:4 and 1:8 dilutions was 84-96%, 81-95% and 89-96% in serum; 80-86%, 82-101% and 88-100% in EDTA plasma; and 87-100%, 80-91% and 82-100% in heparin plasma.

Cyclin B1 (gene symbol CCNB1; UniProt P14635) is a regulatory protein that controls the G2-to-M phase transition of the cell cycle, and its dysregulation has been associated with cancer and developmental disorders. The kit should be stored at 2-8°C for 12 months.