Human CCK-8/Cholecystokinin 8 ELISA Kit

This competitive ELISA from Assay Genie is designed for the accurate quantification of Cholecystokinin-8 (CCK-8) levels in human samples, supporting research into a vital peptide hormone involved in regulating digestion, appetite control, and the release of bile and pancreatic enzymes. Dysregulation of CCK-8 has been implicated in disorders such as obesity, gastrointestinal diseases, and eating disorders, making it a valuable biomarker for exploring these conditions and developing potential interventions.

The kit uses a competitive ELISA format coated with antigen and a colorimetric detection method, with a sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL. It is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity confirmed for human specimens. Reagent storage is at 2-8°C (sealed), and cryopreservation should be avoided. The kit is associated with Uniprot P06307.

Reported linearity across 1:2, 1:4, and 1:8 dilutions is 81-103% / 84-101% / 85-95% for serum (n=10), 81-102% / 81-99% / 83-97% for EDTA plasma (n=10), and 87-105% / 81-99% / 88-100% for heparin plasma (n=10). Recovery ranges and averages are 81-96% (86%) for serum, 81-95% (87%) for EDTA plasma, and 84-100% (93%) for heparin plasma, supporting dependable and reproducible results across a range of research applications.