Human CCAR1 (Cell division cycle and apoptosis regulator protein 1) ELISA KIT (HUFI05376)

This Assay Genie immunoassay kit enables quantitative measurement of Cell Division Cycle and Apoptosis Regulator Protein 1 (CCAR1) in human research samples. CCAR1 is a multifunctional nuclear phosphoprotein that acts as a transcriptional co-regulator of nuclear receptors including the glucocorticoid receptor, modulates p53-dependent apoptosis, and participates in cell-cycle checkpoint control and the DNA-damage response, making it a target of interest in cancer biology, endocrinology, and stress-signalling research.

The product is configured as a complete two-antibody immunoassay, supplied with a pre-coated capture plate, matched detection antibody, enzyme conjugate, calibrated recombinant standard, wash buffer, substrate solution, and stop reagent. Sufficient components are provided for a full plate of individual determinations when used according to the supplied protocol.

Intended applications include profiling CCAR1 abundance in blood-derived research samples, analysing conditioned media from cultured cell lines, and quantifying target levels in cell lysates and tissue homogenates. The product is designated for in vitro research use only and is not validated for clinical or diagnostic decision-making.

Investigators commonly pair CCAR1 protein measurements with parallel mRNA analysis by PCR or western blot validation to confirm transcriptional and translational changes, and store the reconstituted reagents according to the accompanying datasheet to maintain antibody activity across repeated freeze-thaw cycles.