Human CCAAT/enhancer-binding protein beta (CEBPB) ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the quantitative measurement of human CCAAT/enhancer-binding protein beta (CEBPB), also referred to by synonyms including Liver activator protein, Liver-enriched inhibitory protein, Nuclear factor NF-IL6, Transcription factor 5, LAP, LIP, TCF-5, PP9092, C/EBP beta, and TCF5. The assay uses a colorimetric detection method and is reactive with human samples, including serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Specificity covers natural and recombinant human CEBPB.

Reported performance characteristics include a detection range of 78-5000pg/mL and a sensitivity of 39.5pg/mL. Intra-assay CV is 7.3% and inter-assay CV is 10.7%. Linearity across dilutions of 1:2 to 1:16 is as follows: serum 93-103% to 102-112%, EDTA plasma 101-111% to 86-96%, and heparin plasma 102-114% to 91-103%. Mean recovery is 95% (range 89-101%) in serum and 97% (range 91-103%) in plasma.

CEBPB is a key transcription factor involved in regulating the expression of genes related to cell differentiation, inflammation, and immune response, with dysregulation linked to cancer, metabolic disorders, and inflammatory conditions. The kit is associated with UniProt entry P17676, the research area is cancer, and subcellular localisation is in the nucleus and cytoplasm. For research use only.