Human CBS ELISA Kit

From Assay Genie, this colorimetric Sandwich ELISA kit enables quantitative measurement of human Cystathionine Beta-Synthase (CBS) in biological samples. It is reactive with human samples and validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. Sensitivity is reported as 0.938 ng/mL with a detection range of 1.563-100 ng/mL, and the kit is stored at 2-8°C for 12 months.

CBS is an essential enzyme involved in the metabolism of sulfur-containing amino acids, playing a critical role in the synthesis of cysteine and hydrogen sulfide. Dysregulation of CBS activity has been linked to homocystinuria and cardiovascular diseases, highlighting its significance as a biomarker for these disorders. The kit supports research in the Metabolism area.

Reported linearity spans 82-105% across serum, EDTA plasma, and heparin plasma samples at 1:2, 1:4, and 1:8 dilutions (n=5). Recovery ranges from 85-105%, with average recoveries of 97% for serum, 96% for EDTA plasma, and 92% for heparin plasma. The procedure uses a biotin-labeled detection antibody, HRP-Streptavidin (SABC) binding, and TMB substrate, with absorbance read at 450 nm.