Human Cathepsin V ELISA Kit

From Assay Genie, this Human Cathepsin V ELISA Kit is a sandwich ELISA designed to quantify cathepsin V in human samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a double-antibody sandwich format with colorimetric detection.

Reported performance figures include a sensitivity of 18.75 pg/mL and a detection range of 31.25-2000 pg/mL. Linearity in serum (n=5) is 83-100% at 1:2, 91-98% at 1:4, and 84-95% at 1:8; EDTA plasma (n=5) is 80-93%, 88-99%, and 85-96%; heparin plasma (n=5) is 88-101%, 89-98%, and 82-96%. Recovery ranges are 85-104% (average 96%) for serum, 86-103% (average 93%) for EDTA plasma, and 90-94% (average 95%) for heparin plasma. After the stop step, absorbance is measured at 450 nm.

Cathepsin V is a vital enzyme involved in protein degradation and immune response regulation, with dysregulation linked to cancer, autoimmune disorders, and inflammatory conditions. The kit is stored at 2-8°C for 12 months and is associated with the UniProt entry O60911. Listed synonyms include CTSV, CTSL2, CATL2, CTSU, Cathepsin L2, and Cathepsin U.