Human Catechol-O-Methyltransferase (COMT) ELISA Kit

Catechol-O-Methyltransferase (COMT) is a magnesium-dependent intracellular enzyme that catalyzes the O-methylation of catechol substrates, including dopamine, norepinephrine, epinephrine, and a range of hydroxylated metabolites. Because it shapes the clearance rate of catecholamine neurotransmitters and contributes to the inactivation of catechol estrogens, COMT is a frequent target in neuroscience, pharmacogenomics, and steroid metabolism studies.

The Assay Genie Human COMT ELISA Kit is built as a quantitative immunoassay for measuring endogenous COMT protein in human-derived samples. The kit is configured for 96 determinations and supports research workflows that probe enzyme expression at the protein level rather than inferred from transcript data alone. Validated sample matrices include tissue homogenates and cell lysates, with broader applicability to experimental matrices such as conditioned media or cultured cell fractions in comparative studies.

In practical terms, the assay is used by groups working on catecholaminergic signaling, stress and pain modulation, dopaminergic pathway analysis in Parkinson's disease models, and estrogen metabolism research. Each microplate is supplied with the reagents required to run a standard curve and the matched detection antibody set, supporting in vitro research use only. The read-out integrates with standard microplate readers, and the protocol is structured so that laboratories can batch process multiple biological replicates alongside reference samples in a single plate run. Unused reagents should be stored refrigerated to preserve activity between sessions.