Human CAT (Catalase) ELISA Kit (HUFI07676)

Quantitative measurement of human catalase (CAT) protein in serum or plasma is the primary purpose of this ELISA-based kit from Assay Genie. Catalase is a tetrameric heme-containing antioxidant enzyme responsible for decomposing hydrogen peroxide into water and molecular oxygen, and is encoded by the CAT gene on human chromosome 11. The enzyme is widely studied as a biomarker of oxidative stress in clinical and translational research, including investigations of erythrocyte antioxidant status, hepatic function, inflammatory disorders, cardiovascular disease, and age-related degenerative conditions.

The Assay Genie kit is built around an antibody-pair ELISA format, where pre-coated microplate wells capture catalase from the loaded sample. A reporter-conjugated detection antibody binds the captured target, and a chromogenic substrate produces a signal proportional to catalase concentration that is read on a standard plate reader. The kit provides enough reagents for 96 individual determinations, supporting mid-throughput studies in standard laboratory workflows.

This product is intended for research use only and is not cleared for diagnostic procedures. Sample handling recommendations include collecting serum or plasma under standard conditions, aliquoting to avoid repeated freeze-thaw cycles, and storing aliquots refrigerated or frozen until the assay run. Researchers should consult the package insert shipped with the kit for the full protocol, including recommended dilution factors, incubation times, and standard curve preparation steps. Each kit box contains the pre-coated plate, detection reagents, buffers, substrate solution, and a vial of stabilized standard for curve generation.