Human Caspase 9 (CASP9) ELISA Kit

Caspase 9 (CASP9) is a cysteine-aspartic protease that acts as the central initiator caspase of the intrinsic (mitochondrial) apoptotic pathway, so quantitative tracking of its pro-form and cleaved fragments is a standard readout in programmed cell death research. This Assay Genie kit is configured to quantify human CASP9 in the sample matrices most commonly used in apoptosis studies, including tissue homogenates, cell lysates, and cell culture supernatants. The format supports 96 assays per plate, providing enough throughput to compare multiple treatment conditions, time-course points, or drug-dose responses within a single experimental run.

Investigators working on chemotherapeutic response profiling, neurodegeneration models, or intrinsic apoptosis signaling typically pair CASP9 quantification with complementary readouts such as western blot analysis of cleaved CASP9 fragments or downstream caspase-3 activity. For sample handling, aliquoting of freshly prepared lysates before long-term storage is recommended to preserve analyte integrity across freeze-thaw cycles. Processed plates are read on a standard microplate reader in the colorimetric range, and the reagent set is supplied for research use only, supporting in vitro studies of human apoptosis biology rather than clinical diagnostic applications.