Human Caspase 3 (CASP3) ELISA Kit

Caspase-3 is a cysteine-aspartic acid protease that serves as a central executioner of programmed cell death, cleaving structural and regulatory substrates such as PARP during the dismantling of apoptotic cells. The Assay Genie human CASP3 ELISA Kit provides a quantitative colorimetric immunoassay for measuring this protease in cell lysates, tissue homogenates, and cell culture supernatants derived from human samples, supporting research in oncology, neurodegeneration, and compound screening where apoptosis induction is a key readout.

The kit is supplied as a complete microplate-format package containing 96 assays, with all reagents required for the assay workflow including pre-coated wells and detection antibodies specific to human caspase-3. It supports cell culture-based studies that track caspase activation following treatment with chemotherapeutics, kinase inhibitors, or other pro-apoptotic stimuli, and it complements western blot workflows that resolve the 32 kDa proenzyme and its cleaved p17 and p12 active subunits.