Human CASP4 (Caspase 4) ELISA Kit (HUES01819)

From Assay Genie, this Sandwich-ELISA kit is intended for the quantitative measurement of Caspase-4 (CASP4) in human samples, supporting work in serum, plasma, and other biological fluids including cell culture supernatants. The assay uses a colorimetric detection method and follows a standard sandwich workflow: a pre-coated capture antibody binds the target, a biotinylated detection antibody forms the sandwich complex, and an Avidin-Horseradish Peroxidase (HRP) conjugate produces a colour change measured at 450 nm ± 2 nm.

Caspase-4 is described in the product literature as a key enzyme in the inflammatory response pathway, with roles in immune responses and cellular stress, and its dysregulation has been linked to sepsis, inflammatory bowel disease, and neurodegenerative disorders. Stated quantitative performance figures include a sensitivity of 0.1 ng/mL, a detection range of 0.16–10 ng/mL, a sample volume of 100 μL, and a recovery range of 80%–120%. The assay time is listed as 3 h 30 min. Specificity testing reports that the kit recognizes Human CASP4 with no significant cross-reactivity or interference from analogues. Storage is at 2–8°C, and the listed research areas are Cancer, Cell Biology, Metabolism, and Immunology. The associated UniProt identifier is P49662.