Human Cartilage Associated Protein (CRTAP) ELISA Kit

Quantitative measurement of human Cartilage-Associated Protein (CRTAP) in tissue homogenates and cell lysates is supported by this immunoassay kit from Assay Genie. CRTAP operates as part of the prolyl 3-hydroxylation complex that performs post-translational modification of procollagen, a step required for proper collagen triple-helix assembly and extracellular matrix integrity. Mutations disrupting CRTAP cause autosomal recessive osteogenesis imperfecta type VII, so accurate quantification of this protein in experimental specimens is relevant to bone fragility research, skeletal dysplasia studies, and investigations of connective tissue biology.

The kit is configured as a two-antibody immunoassay with absorbance-based readout and includes the matched antibody pairs, standards, and detection reagents required to generate a standard curve and quantify CRTAP across a panel of unknown samples. It is intended for research use only and is compatible with specimens prepared from cultured fibroblasts, osteoblast cell models, and tissue samples processed by homogenization or lysis in standard buffers.

Each package contains material for 96 reactions, supplied in single-use vials together with pre-coated microplate wells. Researchers can apply the kit to compare CRTAP expression between healthy and disease-model tissues, monitor changes following experimental interventions, or characterize cell lines engineered to carry osteogenesis imperfecta-associated mutations.