Human Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit

CPT1A is the liver isoform of carnitine palmitoyltransferase 1, the rate-limiting enzyme for mitochondrial fatty acid β-oxidation. It resides on the outer mitochondrial membrane and transfers long-chain acyl groups from coenzyme A onto L-carnitine, enabling fatty acids to enter the matrix for breakdown. Because the reaction is held in check by malonyl-CoA, CPT1A abundance is a meaningful readout in studies of hepatic lipid metabolism, NAFLD progression, insulin resistance, and mitochondrial bioenergetics.

This Assay Genie ELISA kit is configured for 96 determinations and supplied in microplate format for quantitative measurement of human CPT1A protein. It accepts tissue homogenates and cell lysates as primary sample inputs, and the procedure produces a colorimetric read-out compatible with standard laboratory plate readers. Researchers can apply it to compare CPT1A levels across treated versus untreated hepatocyte cell culture models, model organism liver tissue, or patient biopsy material, supporting work on inherited fatty acid oxidation disorders, drug-induced hepatotoxicity screens, and broader metabolic disease mechanism research.