Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) ELISA Kit

Beyond the headline performance figures, this Assay Genie kit ships as a complete run-ready package: a pre-coated strip plate, lyophilized recombinant CEACAM8 reference standards for an eight-point standard curve, biotinylated detection antibody, streptavidin-HRP conjugate, TMB chromogenic substrate, stop solution, 25-fold wash buffer concentrate, and adhesive plate sealers. The paired-antibody capture workflow—add 50 µL of sample or standard per well, incubate, wash, add detection antibody, wash again, develop with TMB, and read absorbance—lets a full plate be processed in roughly three to four hours on a standard microplate reader.

For neutrophil-biology studies, the assay performs across septic patient serum, bronchoalveolar lavage fluid, and inflamed mucosal biopsies where CD66b is shed during degranulation. Researchers typically report spike-and-recovery values of 85–115% in neat human serum, with intra-assay precision below 10% CV, making it a practical tool for cohort screening in translational inflammation research. Kit components should be refrigerated between uses, with all vials returned to cold storage immediately after handling to preserve the 12-month shelf life; aliquoting the standard after first reconstitution avoids repeated freeze-thaw of the recombinant antigen.