Human Carboxypeptidase B1, Tissue (CPB1) ELISA Kit

Quantitative measurement of tissue carboxypeptidase B1 (CPB1) in human samples is supported by the Assay Genie ELISA Kit. CPB1 is a zinc-dependent metallocarboxypeptidase synthesized by pancreatic acinar cells as the zymogen procarboxypeptidase B1, which is activated in the duodenum where it cleaves C-terminal arginine and lysine residues during protein digestion. Beyond digestive physiology, circulating CPB1 levels have been investigated as biomarkers of pancreatic injury, acute pancreatitis severity, and certain epithelial tumor contexts.

The assay supports standard ELISA applications including biomarker screening, clinical sample profiling, and cell culture supernatant analysis from human-derived in vitro models. Researchers studying pancreatic exocrine function, inflammatory biomarkers, or tumor-associated protease activity will find the kit useful for longitudinal and cohort studies. Storage recommendations should be followed per the product insert, and reagents should be kept refrigerated until first use to preserve enzyme activity and antibody binding performance.

Each kit is supplied with all necessary buffers, standards, and detection reagents to run a full microplate of determinations. The plate-based format allows simultaneous processing of multiple samples, replicates, and standard curve points, supporting robust quantitative analysis. Researchers are advised to review the lot-specific certificate of analysis for any lot-dependent performance notes before planning experiments.