Human Carbonic Anhydrase IV (CA4) ELISA Kit

This Assay Genie ELISA kit enables quantitative measurement of carbonic anhydrase IV (CA4) in research samples, with applications spanning renal and ocular physiology. CA4 is a glycosylphosphatidylinositol (GPI)-anchored zinc metalloenzyme expressed on the apical plasma membrane of renal proximal tubule cells and in corneal endothelium, where it accelerates the reversible hydration of carbon dioxide. Loss-of-function variants in the CA4 gene have been linked to autosomal dominant retinitis pigmentosa (RP17), positioning CA4 as a target of interest in studies of acid-base balance, carbonic anhydrase inhibitor pharmacology, and hereditary retinal disease.

Each kit is supplied as a complete reagent set sized for 96 individual determinations and works with human-derived liquid samples and tissue preparations. Reagents should be stored refrigerated at 4°C; allow the kit to equilibrate to room temperature before running. The procedure uses a standard curve-based assay workflow with detailed plate layout, standard dilution series, and assay buffer preparation provided in the protocol insert. Reconstituted standards and detection antibody should be aliquoted to limit freeze-thaw cycles and preserve signal stability across runs.

Typical applications include biomarker studies in nephrology, ophthalmic research, and characterization of patient-derived material in hereditary retinal dystrophy. The kit is intended for research use only and is not validated for clinical diagnostic procedures.