Human Carbonic anhydrase 3 (CA3) ELISA Kit

Designed by Assay Genie for the quantitative measurement of human Carbonic anhydrase 3 (CA3), this sandwich ELISA kit is intended for research use only. It accepts serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, and is reactive with human samples. Specificity is directed against natural and recombinant human Carbonic anhydrase 3.

Reported performance characteristics include a sensitivity of 0.13ng/mL and a detection range of 0.312-20ng/mL. Intra-assay CV is 6.3% and inter-assay CV is 8.5%. Linearity in serum spans 94-103% at a 1:2 dilution through 89-98% at 1:16, with EDTA plasma at 102-112% to 109-117% and heparin plasma at 108-119% to 96-108% across the same dilution series. Recovery averages 96% (range 90-102) for serum and 98% (range 92-104) for plasma.

Carbonic anhydrase 3 catalyses the reversible hydration of carbon dioxide and is involved in acid-base regulation, respiration and bone resorption. Dysregulation of CA3 has been linked to osteoporosis, glaucoma and certain neurological disorders, making it a biomarker of interest within cell biology research. The protein localises to the cytoplasm.