Human Carbon Catabolite Repression 4 Like Protein (CCRN4L) ELISA Kit

CCRN4L, also known as Nocturnin (NOCT), is a circadian-regulated deadenylase that shortens mRNA poly(A) tails, governing post-transcriptional gene expression with diurnal rhythmicity. The protein has emerged as a key node in studies of adipogenesis, hepatic lipid handling, inflammation, and metabolic homeostasis, and knockout mouse models display altered body weight responses to high-fat diets. Researchers rely on quantitative measurement of CCRN4L to map its tissue-specific oscillation in liver, adipose, and skeletal muscle extracts. This Assay Genie kit supports that workflow for in vitro research use only, with a working calibration range of approximately 0.156–10 ng/mL against the recombinant CCRN4L standard.

Each kit includes a pre-coated microplate and all reagents required for the standard immunoassay procedure, with serial dilutions of the included standard used to build a calibration curve for unknown samples. Load 100 µL of sample per well in duplicate, then follow the sequential incubation and wash steps described in the protocol insert. Unused strip wells and diluted standard should be kept refrigerated between runs; avoid repeated freeze-thaw cycles of reconstituted components to preserve antibody activity. The format accommodates 96 assays per plate, and the supplied protocol insert details reagent preparation, working dilutions, and data interpretation.