Human Carbohydrate Antigen 125 (CA125) ELISA Kit

The Assay Genie CA125 ELISA supports translational workflows focused on MUC16 glycoprotein biology. Ovarian carcinoma cell lines including OVCAR-3 and SKOV-3 are routinely used to characterize antigen shedding into conditioned medium, while serial measurements from patient-derived samples enable calculation of CA125 doubling time and half-life kinetics as pharmacodynamic indicators of therapeutic response. Researchers integrating CA125 with HE4 values can derive Risk of Ovarian Malignancy Algorithm (ROMA) scores for stratifying women presenting with adnexal masses. The capture antibody is human-specific, with no significant cross-reactivity against murine or rat MUC16 orthologs, supporting immunodeficient xenograft studies alongside human sample work. Reconstituted standards and the biotinylated detection antibody should be aliquoted and frozen at -20 °C to limit freeze-thaw cycles, while the streptavidin-HRP conjugate is supplied ready-to-use and should be returned to refrigerated storage immediately after each run. Beyond oncology, elevated serum CA125 concentrations are reported in benign gynecological conditions including endometriosis, pelvic inflammatory disease, and first-trimester pregnancy, so well-controlled comparator cohorts are advisable for biomarker discovery studies.