Human Carbamoyl Phosphate Synthase 1, Mitochondrial (CPS1) ELISA Kit

CPS1 is the rate-limiting mitochondrial enzyme that catalyzes the first committed step of the urea cycle, converting ammonia and bicarbonate into carbamoyl phosphate at the expense of two ATP molecules. It resides in the hepatic mitochondrial matrix and depends on N-acetylglutamate as an essential allosteric activator, so quantitative measurement of CPS1 protein abundance provides a sensitive window into urea-cycle capacity. This Assay Genie kit supports translational research into hyperammonemia, neonatal CPS1 deficiency, acute liver injury, and the mitochondrial metabolic reprogramming observed in hepatocellular carcinoma.

Reconstituted standards and detection antibodies should be stored at -20 °C, while the sealed microplate is kept refrigerated; limit freeze-thaw cycles of working standards to preserve binding activity. The kit accommodates tissue homogenates, cell lysates, and cell culture supernatant samples, giving flexibility across clinical and basic-research workflows. For research use only; not intended for diagnostic procedures.