Human CAR/NR1I3 ELISA Kit

From Assay Genie, this sandwich ELISA kit employs a double-antibody format with colorimetric readout for the quantitative detection of human CAR/NR1I3 (also listed under synonyms including Constitutive Androstane Receptor, NR1I3, CAR1, and MB67). The assay is validated for human samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

Reported performance includes a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Detection uses an HRP-Streptavidin (SABC) system with TMB substrate, and absorbance is read at 450 nm after addition of stop solution. The target is catalogued under UniProt Q14994, and the kit is positioned within the Epigenetics and Nuclear Signaling research area.

Linearity has been characterised for serum, EDTA plasma, and heparin plasma across 1:2, 1:4, and 1:8 dilutions, with reported ranges of 87-103%, 89-104%, and 85-105% for serum; 82-94%, 87-97%, and 82-100% for EDTA plasma; and 81-95%, 81-93%, and 82-97% for heparin plasma. Recovery averages are 96% for serum, 96% for EDTA plasma, and 99% for heparin plasma. Supplied components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled detection antibody, HRP-Streptavidin conjugate, TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer, plate sealer, and a technical manual. Storage is at 2-8°C for up to 12 months.