Human Cannabinoid R1/CB1/CNR1 ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the quantitative measurement of Cannabinoid Receptor 1 (CB1/CNR1) protein in human samples. The kit employs a double antibody sandwich format with colorimetric detection and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Linearity was demonstrated across serum (86-103%, 88-101%, 87-105% at 1:2, 1:4 and 1:8 dilutions), EDTA plasma (91-101%, 86-97%, 82-98%) and heparin plasma (80-99%, 80-97%, 80-97%). Recovery rates were 85-99% (average 92%) for serum, 88-101% (average 96%) for EDTA plasma and 91-99% (average 96%) for heparin plasma. The kit is stored at 2-8°C for 12 months.

The assay protocol includes reagent equilibration to room temperature, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB colour development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution addition. CB1 is a key receptor in the endocannabinoid system involved in appetite regulation, pain perception and memory, and its dysregulation has been implicated in conditions such as obesity, neuropathic pain and addiction.