Human CAND1 (Cullin-associated NEDD8-dissociated protein 1) ELISA KIT (HUFI05339)

Assay Genie's Human CAND1 ELISA Kit enables quantitative measurement of Cullin-associated NEDD8-dissociated protein 1 in human serum and plasma samples, with a sensitivity down to 0.1 ng/mL. CAND1 is a ~136 kDa regulatory protein that binds unneddylated cullins, controlling the assembly and disassembly of cullin-RING E3 ubiquitin ligase complexes central to cell cycle progression, DNA damage response, and protein turnover.

This microplate-based assay uses a TMB substrate for colorimetric readout in research workflows. Each kit provides sufficient reagents for the plate format included, with an assay time of approximately 3 hours. Reagents are stored at -20°C for long-term stability, and serum or plasma samples should be aliquoted and stored refrigerated or frozen to prevent repeated freeze-thaw cycles.

The kit is intended for research use only, supporting investigations into neddylation pathways, SCF complex dynamics, and ubiquitin-mediated proteolysis. Pairing CAND1 quantification with cullin expression profiling can clarify how neddylation status influences ligase activity in disease models.