Human CAMPATH-1 antigen (CD52) ELISA Kit

This sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of CAMPATH-1 antigen (CD52) levels in human samples. CAMPATH-1 antigen, also referred to by synonyms CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5, He5, CDW52, and HE5, is a GPI-anchored cell membrane glycoprotein annotated in UniProt as P31358 and NCBI Gene ID 1043, with a reported molecular weight of 6,614 Da.

Performance characteristics listed by the manufacturer include a colorimetric, sandwich ELISA detection format with a detection range of 0.312-20 ng/mL and a sensitivity of 0.12 ng/mL. Intra-assay CV is reported at 5.2% and inter-assay CV at 9.3%. Specificity is stated for natural and recombinant human CAMPATH-1 antigen. Sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Linearity values for serum, EDTA plasma, and heparin plasma across serial dilutions, along with recovery ranges, are provided in the kit documentation. The research area is immunology, and the product is for research use only.

According to UniProt, the protein may play a role in carrying and orienting carbohydrate, as well as having a more specific role. The manufacturer describes CAMPATH-1 antigen as a key protein in immune responses associated with autoimmune disorders and inflammatory conditions, and notes its relevance to research in multiple sclerosis, rheumatoid arthritis, and transplant rejection.