Human Calponin-1 (CNN1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is intended for the quantitative detection of human Calponin-1 (CNN1), also known as basic calponin or calponin H1, smooth muscle. Calponin-1 is a thin filament-associated protein implicated in the regulation and modulation of smooth muscle contraction, capable of binding to actin, calmodulin, troponin C and tropomyosin, and belongs to the calponin protein family.

Manufacturer-reported performance figures include a detection range of 0.312-20ng/mL, a sensitivity of 0.078ng/mL, an intra-assay CV of 4.7%, and an inter-assay CV of 9.3%. The assay is reactive with human samples, including serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and is specific for natural and recombinant human Calponin-1. UniProt accession: P51911. The kit supports research applications in cardiovascular studies, including investigations related to hypertension and smooth muscle disorders.

Reported linearity at a 1:2 dilution is 103-113% in serum (n=5), 105-114% in EDTA plasma (n=5), and 99-109% in heparin plasma (n=5). Spike-and-recovery averages are 103% for serum (range 97-109%) and 105% for plasma (range 99-111%). The kit is supplied for research use only; reagent storage temperatures range from -20°C to 4°C as listed for individual components.