Human Calpain-3 (CAPN3) ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of human Calpain-3 (CAPN3), a calcium-regulated non-lysosomal thiol-protease also known by synonyms such as Calpain p94, Calpain L3, CANP 3, nCL-1, and Muscle-specific calcium-activated neutral protease 3 (UniProt P20807). The assay is reactive with human samples and is intended for research use only.

Specifications quoted from the manufacturer include a detection range of 0.312–20 ng/mL and a sensitivity of 0.164 ng/mL, with the assay read on a micro-plate reader at 450 nm. The kit is suitable for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and shows specificity for both natural and recombinant human Calpain-3.

Calpain-3 is described in the product literature as interacting with TTN/titin and with CMYA5, the latter interaction potentially protecting CAPN3 from autolysis. The protein localises to the cytoplasm, and defects in CAPN3 are reported to cause limb-girdle muscular dystrophy type 2A (LGMD2A). The listed research area for this product is cardiovascular.