Human Calpain-1 catalytic subunit (CAPN1) ELISA Kit

From Assay Genie, this sandwich-format assay is designed for quantitative measurement of the human Calpain-1 catalytic subunit (CAPN1). The kit is for research use only and reacts with human samples, with stated specificity for natural and recombinant human Calpain-1 catalytic subunit. Sample types listed in the datasheet are serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Reported performance figures include a detection range of 0.312-20ng/mL and a sensitivity of 0.156ng/mL. Precision is stated as an intra-assay CV of 6.0% and an inter-assay CV of 9.6%. Spike-and-recovery testing gave an average of 101% (range 95-107%) for serum and 103% (range 97-109%) for plasma. Dilutional linearity at 1:2, 1:4, 1:8 and 1:16 was 93-93%, 95-104%, 103-116% and 88-99% in serum (N=5); 102-114%, 102-111%, 101-113% and 113-122% in EDTA plasma (N=5); and 107-116%, 112-121%, 80-88% and 85-95% in heparin plasma (N=5).

CAPN1 is described as a calcium-regulated non-lysosomal thiol-protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction, and forms a heterodimer with the small regulatory subunit CAPNS1. Listed synonyms are Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, CANP 1, muCANP, PIG30 and CANPL1. The UniProt accession is P07384 and the research area listed in the datasheet is Neurosciences.