Human Calmodulin ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the in vitro quantitative determination of human CALM1 (calmodulin) concentrations in serum, plasma, and other biological fluids, including cell culture supernatants, urine, cerebrospinal fluid, cell lysates, and tissue homogenates. Calmodulin is a key calcium-signalling protein involved in muscle contraction, neurotransmitter release, and gene expression, and its dysregulation has been linked to cardiac arrhythmias, neurodegenerative disease, and cancer, making it a valuable biomarker for research.

Detection uses a colorimetric, double-antibody sandwich method. Sensitivity is reported at 56.25 pg/ml with a measuring range of 93.75–6000 pg/ml. Validation data show intra-assay CV below 8% and inter-assay CV below 10%. Spike-and-recovery in serum averaged 96% (range 87–101%), in EDTA plasma 93% (87–101%), and in UFH plasma 98% (90–102%). Linearity was confirmed across serial dilutions in the same matrices. The kit is intended for research use only.

Supplied components include a dismountable 8×12-strip microplate, lyophilised standard, sample/standard dilution buffer, biotin-labelled antibody, antibody dilution buffer, HRP-streptavidin conjugate, SABC dilution buffer, TMB substrate, stop solution, 25X wash buffer, and plate sealer. Most components are stored at 4 °C for six months; the lyophilised standard is stored at 4 °C or −20 °C, and light-sensitive reagents should be protected from light. UniProt reference: P0DP23. The published protocol uses sequential 37 °C incubations with biotinylated detection antibody and SABC working solution, TMB substrate development, and absorbance reading at 450 nm.