Human CALCOCO2 (Calcium-binding and coiled-coil domain-containing protein 2) ELISA Kit (HUFI04600)

This quantitative immunoassay kit from Assay Genie is purpose-built for measuring endogenous levels of CALCOCO2 (Calcium-binding and coiled-coil domain-containing protein 2), a 52 kDa cytosolic adaptor also known as NDP52 that mediates selective autophagy, xenophagy, and innate immune signaling through recognition of ubiquitin-coated bacteria and damaged mitochondria.

The colorimetric plate-based format is calibrated for use with human serum samples and corresponding plasma specimens, providing a reproducible read-out on a standard microplate reader. The 52 kDa target is captured between a pre-coated capture antibody and a biotinylated detection antibody, with streptavidin-HRP and TMB substrate delivering the colorimetric signal. Each kit contains pre-coated wells, lyophilized recombinant standards, detection reagents, wash buffer concentrate, and a complete assay buffer set, supporting an end-to-end laboratory workflow from sample loading through data analysis.

Researchers studying TBK1 phosphorylation events, SQSTM1/p62 crosstalk, or pathogen clearance mechanisms will find this kit useful for cell lysate, tissue homogenate, and related fluid investigations. The pack provides reagents for 96 individual determinations in a single-use microplate configuration. Store reagents refrigerated until first use, and follow the protocol insert for guidance on the long-term stability of diluted working solutions. Intended for research use only.