Human Calcium-binding mitochondrial carrier protein Aralar1 (SLC25A12) ELISA Kit

A high-quality sandwich ELISA kit from Assay Genie designed for the quantitative detection of human Calcium-binding mitochondrial carrier protein Aralar1 (SLC25A12). The kit targets the SLC25A12 protein, also referred to as Mitochondrial aspartate glutamate carrier 1, Solute carrier family 25 member 12, ARALAR1, AGC1, ARALAR and EIEE39, and is intended for research use only.

The assay reports a detection range of 0.312–20 ng/mL and a sensitivity of 0.1 ng/mL, with an intra-assay CV of 4.2% and an inter-assay CV of 7.8%. Linearity has been evaluated in serum (107–117%, 109–119%, 91–99%, 89–97% across 1:2 to 1:16 dilutions), EDTA plasma (108–117%, 106–116%, 81–90%, 109–117%) and heparin plasma (100–110%, 104–117%, 108–118%, 89–99%). Recovery in serum averages 88% (range 82–94%) and in plasma averages 90% (range 84–96%). Specificity is reported for natural and recombinant human Calcium-binding mitochondrial carrier protein Aralar1.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. According to the cited UniProt entry (O75746), the target protein catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane and may have a function in the urea cycle. The protein localises to the mitochondrion inner membrane as a multi-pass membrane protein and is classified under the Signal Transduction research area. The kit is supplied with the components required to perform the assay, including microplate strips, lyophilized standard, sample and assay diluents, detection reagents, wash buffer, substrate and stop solution.