Human Calcineurin subunit B type 1 (PPP3R1) ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of human Calcineurin subunit B type 1 (PPP3R1) in research samples, supporting studies of calcineurin-mediated signal transduction, gene expression and immune response regulation. Also known as Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1, CNA2 and CNB, PPP3R1 is the calcium-sensitive regulatory subunit of the calcineurin phosphatase complex, and its dysregulation has been implicated in autoimmune disorders, neurodegenerative disease and certain cancers.

The assay uses a colorimetric ELISA detection method with reactivity limited to human samples. Reported performance figures include a detection range of 0.156-10ng/mL, a sensitivity of 0.067ng/mL, an intra-assay CV of 8.4% and an inter-assay CV of 10.1%. Specificity is defined against natural and recombinant human Calcineurin subunit B type 1, and the kit is positioned within the Neurosciences research area.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The protein (UniProt P63098) localises to the cytoplasm, cytosol, cell membrane and sarcolemma, and translocates from the cytosol to the sarcolemma in a CIB1-dependent manner during cardiomyocyte hypertrophy. The kit is supplied for research use only.