Human Cadherin-17 PharmaGenie ELISA Kit (SBRS0405)

Quantitative measurement of human Cadherin-17 (CDH17) in cell culture supernatants and blood-derived research samples is supported by the PharmaGenie kit from Assay Genie. Cadherin-17, also called LI-cadherin, is a calcium-dependent cell adhesion molecule that is normally restricted to intestinal and gastric epithelium. Re-expression of CDH17 has been documented in several gastrointestinal and hepatic tumor types, making it a useful biomarker in oncology studies of gastric, colorectal, and hepatocellular carcinoma, as well as in research on tumor invasion, intestinal epithelial biology, and metastasis.

The kit is supplied in a microplate format and produces a colorimetric readout that can be read on a standard laboratory plate reader. Each kit ships with pre-coated wells, capture and detection reagents, a set of reference standards, wash buffer, substrate, and ancillary reagents sufficient for the indicated number of assays. The workflow follows a stepwise incubation procedure: standards and samples are added to coated wells, plates are washed, detection reagent is applied, and a substrate step generates a measurable signal. CDH17 concentrations in unknown samples are interpolated from a standard curve generated using the kit's calibrators.

For best results, kit components should be stored refrigerated upon receipt, and reconstituted reagents should be aliquoted to limit repeated freeze-thaw cycles that can degrade assay performance. The kit is intended for research use only and is suitable for studies characterizing CDH17 regulation in cell-based models, primary tissue research, and translational gastrointestinal cancer investigations.