Human C9orf140 ELISA Kit

A competitive ELISA kit from Assay Genie enables quantitative detection of human C9orf140 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a coated-antigen format with colorimetric detection, delivering a sensitivity of 0.469 ng/mL and a working range of 0.781–50 ng/mL. The target protein corresponds to UniProt Q86UD0 and is also known as TS, MDEP, tumor specificity and mitosis phase-dependent expression protein, and p42.3, supporting research in the cell cycle area. C9orf140 has been described as playing roles in gene regulation and cell signaling, with abnormal levels linked to several diseases, making it a useful biomarker for studying disease pathogenesis and exploring potential therapeutic targets.

Reported linearity data (n = 10) across 1:2, 1:4, and 1:8 dilutions cover 85–105% for serum, 83–100% for EDTA plasma, and 82–99% for heparin plasma. Recovery ranges are 89–98% for serum, 92–105% for EDTA plasma, and 88–104% for heparin plasma, with corresponding averages of 95%, 100%, and 96%. The procedure uses a biotinylated antibody binding step followed by TMB substrate development and absorbance measurement at 450 nm. Reagents are stored at 2–8°C and should not be cryopreserved.